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Isopropyl-beta-D-thiogalactopyranoside(IPTG) ; CAS NO.: 367-93-1

Isopropyl-beta-D-thiogalactopyranoside(IPTG) ; CAS NO.: 367-93-1

CAS NO.: 367-93-1
EINECS: 206-703-0
Synonyms: YPD BROTH E; Isopropyl 2,3-O-isopropylidene-4-O-benzoyl-β-L-thiorhamnopyranoside; IPTG (Isopropyl-β-D-thiogalatopyranoside); 1-ISOPROPYL-BETA-D-THIOGALACTOPYRANOSIDE; isopropyl-thiogalactopyranoside; 1-methylethyl1-thio-.beta.-D-Galactopyranoside
MF: C9H18O5S
MW: 238.3
Melting Point: 105°C

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Description

Character of Isopropyl-beta-D-thiogalactopyranoside(IPTG) | CAS NO.: 367-93-1

 

White Crystalline or Crystalline powder,odorless , dissolved in water and methanol

 

Test Item

Specifications

Appearance

White Crystalline

Solubility (Concentration)

49.00-55.00mg/mL

Solution clarify and color

Clarify, colorless

Purity (by HPLC,% area)

≥99%

Moisture(K.F.)

<1.0%

NMR (D2O)

Consistent with Structure

Residual Detection of Dioxane Ring (GC)

Not Contain

Specific Rotation

-28.00°~ -33.00°

 

Product Introduction

 

Application field

Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective β-galactosidase inducer of protein expression where the gene is under the control of the lac operator. It is used with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside to identify the non-recombinant plasmid in cloning experiments by blue-white screen methodology.

Product Brief

 IPTG (Isopropyl-β-D-thiogalactopyranoside) is a galactose analogue not recognized by β-galactosidase. IPTG is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. It is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of β-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Package

1kg/Aluminium Foil Bag, or as per client's requirement

Storage conditions

2-8°C;Store in originally sealed packaging in a cool and dry place

Shelf life

2 years

 

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Our products are formulated to meet the needs of all skin types.

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We will uphold the enterprise spirit of "honesty, dedication, efficiency and innovation", concentrate our efforts, overcome difficulties, continuously shape the advantages of the enterprise, enhance competitiveness and accelerate the pace of development.

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As a supplier and manufacturer, we offer competitive prices.

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We do a good job in wages and welfare according to the requirements of the modern enterprise system, formulate distribution policies with incentive effect and coordinate labor-management relations.

5

Our ingredients are formulated to be compatible with different pH levels.

6

We are fortunate to be in the tide of economic development in China and the world, rooted in the industry, and committed to creating high-quality, low-cost, energy-saving and environmentally friendly Isopropyl-beta-D-thiogalactopyranoside(IPTG) CAS NO. 367-93-1

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The company always adheres to the tenet of 'honesty, quality for life, innovation as the subject, development-oriented', abides by the quality commitment to users, pays attention to the introduction of talents, so as to offer the best products to customers around the world.

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Our products are thoroughly tested and inspected before leaving the factory.

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We always adhere to the principle of efficiency first and take the path of high quality development.

 

FAQ

 

Q: What is the benefit of Isopropyl-beta-D-thiogalactopyranoside(IPTG) ?

A: IPTG (also known as Isopropyl-β-D-Thiogalactopyranoside) is a molecular biology reagent that functions as an inducer of galactosidase activity by binding to and inhibiting the repressor. It is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.IPTG is often used at a final concentration ranging from 0.5-1.0 mM. X-Gal is often used with IPTG since it provides an easy visual method to monitor whether protein expression has occurred. X-Gal as the name implies contains a galactose group that once metabolized causes a bright blue color change.

Q: Where is Isopropyl-beta-D-thiogalactopyranoside(IPTG) found

A: Isopropyl beta-D-thiogalactopyranoside is an S-glycosyl compound consisting of beta-D-1-thiogalactose having an isopropyl group attached to the anomeric sulfur.

 

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